Protein Expression Testing
AKTA FPLC (ion-exchange, IMAC)
Protein Purification Process Development
Non-Tagged Protein Purification
Complete Batch Records
Manufacturing Pilot Process
Scale-Up
Custom Protein Formulations
Tissue Culture (primary cells, adherent and suspension cell lines)
Viability Assays (fluorescent, colormetric)
Custom Competent Cell Preparation
(chemical, electrocompetent, 96 well
format)
(chemical, electrocompetent, 96 well
format)
Subcloning
Bacterial or Yeast Growth
Custom Protein Formulations
Thermophilic DNA polymerases
Phi29 DNA polymerase
MMLV and AMLV reverse transcriptase
T4 DNA Ligase
Taq DNA Polymerase
| Cat. number | Concentration (U/ul) | Size (ul) | Size (units) | Price (US Dollar) | |
| 2001 | 5 | 100 | 500 | $55.00 |  |
| 2002 | 50 | 100 | 5,000 | $350.00 | |
| 2003 | 5 | 1000 | 5,000 | $350.00 | |
You can also place an order by faxing a completed order form to 858-558-3740 or calling us at 858-558-3702.
Description:
This is a wild-type recombinant DNA polymerase, derived from T. aquaticus, expressed in E. coli. The Taq polymerase is a thermo-stable DNA polymerase with 5' to 3' polymerase activity and low 5' to 3' exonuclease activity.
Available in two concentrations and supplied with 10X reaction buffer.
The higher-concentration (50 U/µl) enzyme is also supplied with 1X dilution buffer.
Protein Uses:
Primer extension.
Polymerase chain reaction.
DNA sequencing.
Site-directed mutagenesis.
Additional Information:
Single recombinant polypeptide of 94 kDa, expressed in E.coli.
High purity, low DNA content.
Specific activity: 100,000 - 150,000 U/mg.
Exhibits deoxynucleotidyl-transferase activity, which results in the addition of extra adenines at the 3' end of PCR products - suitable for use in TA cloning.
Half-life of 45 minutes @ 94°C.
Error rate of 1/45,000 bases.