Product Description
Item # 2004

Product/Protein : 2x MidasMix with Thermus aquaticus DNA Polymerase I
Lot # : 030507BM
Concentration : 200 reactions, 250 units/ml
Package format : 2000µl = 4x 500 µl Midasmix. This all-in-one reaction mix is 2x in reaction buffer and nucleotides. All you need to add is your primer/template mix in an equal volume of water.
1X Reaction Buffer: 75mM Tris pH 8.8
2mM MgCl2
 20mM (NH4)2SO4
0.01%v/v Tween 20
0.22mM dNTP
125 units/ml Taq Polymerase
Description : This convenient, easy to use 2x formulation combines Taq DNA polymerase , buffer and all of the reaction components required for Taq mediated DNA amplification . The 2x formulation allows complete control of final reaction volume. Simply add template and primers mix and amplifyi.
Protein Uses : Primer extension (2) , polymerase chain reaction (1,5) ,DNA sequencing (3,4) and site-directed mutagenesis.
Properties : The enzyme features strand displacement capability , an error rate of one in @4.5x104 bases and has low 5’-3’ exonuclease activity. Like other DNA polymerases without 3’-5’ exonuclease activity, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which results in the addition of extra adenines at the 3'- end of PCR products.

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Product Quality Assurance Verification
Item # 2004

Protein/Product :

 2x MidasMix with Thermus aquaticus DNA Polymerase I ug reduced - protein quality assurance

Concentration :

 200 reactions, 250 units/ml

Reaction Conditions :

 75mM Tris pH 8.8
2mM MgCl2
20mM (NH4)2SO4
0.01%v/v Tween 20
0.22mM dNTP’s
125 units/ml Taq Polymerase

Quality Assessment

Assay

Result
dsDNA endonuclease Degrade ΦX174 RF None
Detected
ssDNA endonuclease Degrade M13mp18 None
Detected  
5' dsDNA exonuclease Removal of labeled nucleotide from 5' end of a dsDNA oligonucleotide None
Detected  
5' ssDNA exonuclease Removal of labeled nucleotide from 5' end of a ssDNA oligonucleotide Active  
3' ssDNA+dsDNA exonuclease Removal of labeled nucleotide from 3' end of a ssDNA or a dsDNA oligonucleotide None
Detected  
unit functional assay 1 unit incorporates 10nMol 32P dCTP into acid insoluble material in 30 minutes at 74°C using salmon testes DNA as substrate Greater than 100,000 units/mg protein  
  1. Cha, R.S. and Thilly, W.G. (1995) in: PCR Primer, Dieffenbach, C.W. and Dveksler, G.S. (eds.), CSH Press, New York, 37..
  2. Eckert KA, and Kunkel TA. Nucleic Acids Res. (1990), 18(13), 3739-44.
  3. Ishino,Y et al. (1994) J. Biochem. 116 (5), 1019-1024
  4. Kusukawa N et al. Biotechniques. 1990 9(1), 66-8, 70, 72.
  5. Schmidt T. M. et al. Biotechniques. (1991) 11(2):176-7
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