Protein Description
Item # 2001

Protein : Thermus aquaticus DNA Polymerase ; recombinant
Lot # : 112706BM
Concentration : 5,000 units/ml ; 0.038 mg/ml
Package format : 100µl = 500 units enzyme. Also included is 1.0ml 10X reaction buffer.
10X Reaction Buffer: 750mM Tris pH 8.8
20mM MgCl2
 200mM (NH4)2SO4
0.1%v/v Tween 20
Description : Taq DNA polymerase is a thermostable DNA polymerase with 5'-3' polymerase activity and low 5'-3' exonuclease activity . This protein is wild-type, recombinant DNA polymerase derived from Thermus aquaticus and expressed in E.coli .
Protein Uses : Primer extension (2), polymerase chain reaction (1,5), DNA sequencing (3,4) and site-directed mutagenesis.
Properties : The enzyme features strand displacement capability , an error rate of one in 4.5x104 bases and has low 5'-3' exonuclease activity. Like other DNA polymerases without 3'-5' exonuclease activity, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which results in the addition of extra adenines at the 3'-end of PCR products.

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Protein Quality Assurance Verification
Item # 2001

Protein :

 Thermus aquaticus DNA Polymerase ; recombinant ug reduced - protein quality assurance

Concentration :

 5,000 units/ml ; 0.038mg/ml

Formulation :

 20mM Tris pH 8.0
0.1mM EDTA
1mM DTT
0.5% Igepal Ca-630
0.5% Tween 20
50% v/v Glycerol

Quality Assessment

Assay

Result
dsDNA endonuclease Degrade ΦX174 RF None
Detected
ssDNA endonuclease Degrade M13mp18 None
Detected  
5' dsDNA exonuclease Removal of labeled nucleotide from 5' end of a dsDNA oligonucleotide None
Detected  
5' ssDNA exonuclease Removal of labeled nucleotide from 5' end of a ssDNA oligonucleotide Active  
3' ssDNA+dsDNA exonuclease Removal of labeled nucleotide from 3' end of a ssDNA or a dsDNA oligonucleotide None
Detected  
unit functional assay 1 unit incorporates 10nMol 32P dCTP into acid insoluble material in 30 minutes at 74°C using salmon testes DNA as substrate Greater than 100,000 units/mg protein  
  1. Cha, R.S. and Thilly, W.G. (1995) in: PCR Primer, Dieffenbach, C.W. and Dveksler, G.S. (eds.), CSH Press, New York, 37..
  2. Eckert KA, and Kunkel TA. Nucleic Acids Res. (1990), 18(13), 3739-44.
  3. Ishino,Y et al. (1994) J. Biochem. 116 (5), 1019-1024
  4. Kusukawa N et al. Biotechniques. 1990 9(1), 66-8, 70, 72.
  5. Schmidt T. M. et al. Biotechniques. (1991) 11(2):176-7
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