E.coli DNA polymerase I, Klenow fragment, 3'-5' exonuclease negative mutant. Bacterial expression, Low DNA.

Additional Information:

• Recombinant single protein; not a fusion construct. 
• Generates probes using direct or indirect labeling methods.
• Specific Activity: 15,000-20,000 units/mg.
• Low DNA content
• High concentration, automation grade.
• Supplied with 10X Reaction Buffer. High concentrations supplied with 1X Dilution Buffer

Description:
Klenow Fragment (3´» 5´ exo- ) is an N-terminal truncation of  E.coli DNA Polymerase I. This tuncation deletes the domain containing the 5´» 3´ exonuclease activity leaving the polymerase activity intact.  Klenow Fragment (3´» 5´ exo- ) also has two mutations (D355A, E357A) which abolish the 3´» 5´ exonuclease activity (1). Our high concentration preparations are suitable for robotic applications and all formulations feature low DNA content.