Bacteriophage T4 DNA Ligase

Additional Information:

• Recombinant single polypeptide of @ 55.3kDa
• Joins double-stranded DNA with cohesive or blunt termini
• Attaches oligonucleotide linkers or adaptors to blunt-ended DNA
• Repairs nicks in duplex DNA, RNA or DNA-RNA hybrids
• Specific Activity: ≥ 400,000units/mg
• Low DNA content
• Supplied with a 10X Reaction Buffer and a 5X PEG Reaction Buffer

Description:

Bacteriophage T4 DNA Ligase

is derived from Eschericia coli bacteriophage T4 and expressed in E.coli. This DNA ligase catalyzes the formation of a phosphodiester bond between proximal 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive-end termini. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-stranded nucleic acids . The T4 DNA Ligase requires ATP as cofactor. This

highly purified

  preparation has low contaminating bacterial DNA and is suitable for construction of linker-tailed genomic DNA libraries for PCR and use in emulsion PCR techniques.